Journal: Redox Biology
Article Title: Pyrogallol intermediates elicit beta-amyloid secretion via radical formation and alterations in intracellular trafficking, distinct from pyrogallol-generated superoxide
doi: 10.1016/j.redox.2024.103180
Figure Lengend Snippet: Changes of other ROS parameters except DHE fluorescence by PG treatment in 293sw cells. (A–D) Analysis of relative DCF fluorescence (A), HPF fluorescence (B), EtS-DMAB fluorescence (C), and PO-1 fluorescence (D) of cell-free media (left panel) and the cells (right panel) after 1 h or 6 h treatment indicated doses of PG. ( E –I) Comparison of effects on the ROS parameters by PG, rotenone, and H 2 O 2 in cell-free media and 293sw cells. 50 μM PG, 2 μM rotenone, and 0.5 mM H 2 O 2 were treated into cell-free media (left panel) and the cells (right panel) for 1 h or 6 h, and relative DHE fluorescence (E), DCF fluorescence (F), HPF fluorescence (G), EtS-DMAB fluorescence (H), and PO-1 fluorescence (I) were analyzed. Values of the relative fluorescence are represented compared with PG-untreated group in panel A, D, E, F, and I or with blank value in panel B, C, G, and H. Statistical significances were determined using Student's t-test compared with the untreated control (n = 3, *p < 0.05, **p < 0.01).
Article Snippet: Hydrogen peroxide (H 2 O 2 ), Hydroxyl Radical and Peroxynitrite Sensor (HPF), peroxy orange 1 (PO-1), and EtS-DMAB (HClO-green) were sourced from Junsei Chemical (Japan), Invitrogen (USA), Tocris (USA), and MedChemExpress (MCE, USA) respectively.
Techniques: Fluorescence, Comparison, Control